Figure 8.

The specific autophagy receptors SQSTM1, OPTN, and CACO2 aggregate in response to peroxisome-derived H₂O₂ production. DD-DAO Flp-In T-REx 293 cells were cultured as described in the legend to Figure 3. The cells were subsequently transferred to DBPS containing 10 mM 3-amino-1,2,4-triazole (3-AT) and 10 mM L- or D-Ala. After 60 min, the cells were fixed and processed for immunofluorescence microscopy using rabbit antisera against SQSTM1, OPTN, or CACO2 in combination with a goat anti-rabbit secondary antibody conjugated to Texas Red. The panels shown on the right of each image are magnified views of the boxed areas. Scale bars, 10 μm.