Figure 14.

Expression of OPTN-GFP triggers pexophagy. DD-DAO Flp-In T-REx 293 cells expressing po-mKeima were first transfected with a plasmid encoding GFP or OPTN-GFP and subsequently cultured in rMEMα. Two days later, the cells were processed for live-cell imaging. (A) Representative fluorescence images of (OPTN-)GFP (false color: blue) and po-mKeima on excitation at 440 (false color: green) and 586 (false color: red) nm. Scale bar, 10 µm. (B) Random fields of view were selected in the bright field, and the 586/440 nm fluorescence excitation ratios of po-mKeima were measured and plotted. **, p < 0.01.