Figure 4.

NKG2D deficiency leads to a reduced IFNγ response in Apc^min/+ mice. (a) Representative flow cytometry plots of tumor-infiltrating IFNγ-producing CD8⁺ T cells in Apc^min/+Klrk1^+/+ and Apc^min/+Klrk1^−/− mice at disease endpoint. (b) Percentages of tumor-infiltrating CD8⁺ T cells in Apc^min/+Klrk1^+/+ and Apc^min/+Klrk1^−/− mice at disease endpoint. (c) Percentages of tumor-infiltrating IFNγ⁺ cells within the CD8⁺ T cell subset in Apc^min/+Klrk1^+/+ and Apc^min/+Klrk1^−/− mice. (d) Categories of IFNγ levels in Apc^min/+Klrk1^+/+ compared to Apc^min/+Klrk1^−/− mice. (e) Percentages of tumor-infiltrating PD-1 expressing CD8⁺ T cells in Apc^min/+Klrk1^+/+ compared to Apc^min/+Klrk1^−/− mice. Statistical significance was determined using Mann Whitney U or unpaired t-test following a Shapiro–Wilk normality test (b,c,e) or Chi-square (d). Bars represent mean ± SEM. * p ≤ 0.05.