Table 1.

Primer sequences and amplification programs.

Gene	Primer Sequences	Conditions	Product Size (bp)
IL-6	5′-ATGAACTCCTTCTCCACAAGCGC-3′ 5′-GAAGAGCCCTCAGGCTGGACTG-3′	5″ at 95 °C, 13″ at 56 °C, 25″ at 72 °C for 40 cycles	628
IL-8	5′-ATGACTTCCAAGCTGGCCGTG-3′ 5′-TGAATTCTCAGCCCTCTTCAAAAACTTCTC-3′	5″ at 94 °C, 6″ at 55 °C, 12″ at 72 °C for 40 cycles	297
IL-1α	5′-CATGTCAAATTTCACTGCTTCATCC-3′ 5′-GTCTCTGAATCAGAAATCCTTCTATC-3′	5″ at 95 °C, 8″at 55 °C, 17″ at 72 °C for 45 cycles	421
TNF-α	5′-CAGAGGGAAGAGTTCCCCAG-3′ 5′-CCTTGGTCTGGTAGGAGACG-3′	5″ at 95 °C, 6″ at 57 °C, 13″ at 72 °C for 40 cycles	324
TGF-β	5′-CCGACTACTACGCCAAGGAGGTCAC-3′ 5′-AGGCCGGTTCATGCCATGAATGGTG-3′	5″ at 94 °C, 9″ at 60 °C, 18″ at 72 °C for 40 cycles	439
HBD-2	5′-GGATCCATGGGTATAGGCGATCCTGTTA-3′ 5′-AAGCTTCTCTGATGAGGGAGCCCTTTCT-3′	5″ at 94 °C, 6″ at 63 °C, 10″ at 72 °C for 50 cycles	198
Occludin	5′-TCAGGGAATATCCACCTATCACTTCAG-3′ 5′-CATCAGCAGCAGCCATGTACTCTTCAC-3′	10″ at 95 °C, 45″ at 60 °C for 40 cycles	188
Zonulin-1	5′-AGGGGCAGTGGTGGTTTTCTGTTCTTTC-3′ 5′-GCAGAGGTCAAAGTTCAAGGCTCAAGAGG-3′	10″ at 95 °C, 45″ at 60 °C for 40 cycles	217
Claudin-1	5′-CTGGGAGGTGCCCTACTTTG-3′ 5′-ACACGTAGTCTTTCCCGCTG-3′	1″ at 95 °C, 30″ at 60 °C, 20″at 72 °C for 40 cycles	128