Figure 3.

Compatibility of CFP-10 capture and detection antibody pairs. The capture and control antibodies were dotted on the nitrocellulose membrane using 1 µL of 1 mg/mL antibody. The membranes were left to dry for 15 min prior to testing. To test the samples, the negative control contained 30 µL of running buffer and 10 µL of AuNP–antibody conjugate, whilst the test sample also contained recombinant CFP-10 protein (4 µg/mL) or ESAT-6 protein (2.7 µg/mL). Tests were allowed to run for 15 min before analysis. ‘Ghost lines’ are indicated by blue circles.