Scheme 2.

Schematic diagram showing steps of the assembling and wet testing of LFIA strips. The diagram illustrates the lamination of components of a strip on the backing card (steps 1 to 4). (1) The nitrocellulose membrane is the first to be laminated, followed by (2) the conjugate and sample pads which have a 2 mm overlap, and finally (3) the absorbent pad is embedded on the card. Following the cutting of the strips, wet testing (step 4) was carried out as shown on the diagram. Lines on both control and test lines represent a positive test, while a single line in the control represent a negative test.