Table 2.

Comparison of various electrochemical biosensors.

Sr. No.	Composition of Electrode	Detection Method	Method of Immobilization	LOD (µM)	Linear Range (µM)	Optimum pH	Optimum Temperature (°C)	Response Time (Second)	Storage Life (Days)	Samples	References
1	AChE-ChO/GO-IL/GCE	ADPSV	Adsorption	8.85 × 10−4, 1.352 × 10−3	5 × 10−3–1 × 10−6	7.4	NR	NR	90	Serum	[7]
2	AChE-ChO/GO-AuNPs-CS/Fe3O4–TiO2@NH2/GCE	ECL	Cross-linking	0.002.2	6.7 × 10−3 –0.92 × 103	8	NR	NR	15	Serum	[8]
3	AChE-ChO/QDs/rGO/GCE	ECL, CV	Cross-linking	8.8, 4.7	10–210, 10–250	9.0	NR	NR	7	Serum	[9]
4	AChE-ChO/a PDDA/ZnO/MWCNTs/PGE	CV	Adsorption	0.3	1.0–0.8 × 103, 1.0–103	NR	50	60	90	Serum	[10]
5	AChE-ChO/c PPy-PVS/Pt	CV	Cross-linking	5.0 × 10−3	10−5–10−3	9.0	65	200	NR	Artificial blood	[11]
6	AChE-ChO/MWCNT-MnO2/rGO/Au	CV	Cross-linking	0.1	0.1–1.00	7.4	35	NR	90	Serum	[12]
7	AChE-ChO/MOF/PtNPs/Au	DPV	Covalent attachment	0.01	0.01–500	7.4	30	NR	120	Serum	[13]
8	AChE-ChO/CS/Fe@AuNPs/Au	SWV	Cross-linking	5 × 10−2	5.0 × 10−3–400	7.0	30	3	90	Serum	[14]
9	AChE-ChO/rGO/PtNP/ITO	CV	Cross-linking	5 × 10−3	5.0 × 10−3−700	7.0	35	4	120	Serum	[15]
11	AChE-ChO/Fe2O3/rGO/PEDOT/FTO	EIS	Cross-linking	4.0 × 10−3	0.004–800	7.0	30	3	90	Serum	[16]
12	AChE-ChO/AuNPs/pTTB/SPCE	CA	Covalent attachment	0.0026	0.7 × 10−3 −1.5 × 103	NR	NR	NR	NR	Serum	[17]
13	AChE-ChO/CDs-APTES/CPE	CV	Cross-linking	5.0 × 10−3	10−5−10−2	7.0	60	200	NR	Artificial blood	[18]
14	AChE/PANI-Nano-ZSM−5/GCE	SWV	Entrapment	0.1	1.0–103	7.4	NR	NR	15	-	[33]
15	AChENPs/ChONPs/GONS/PtNPs/PGE	CV	Adsorption	0.001	0.001–200	7.5	35	3	180	Serum	This work