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. 2023 Mar 10;15(6):1715. doi: 10.3390/cancers15061715

Figure 1.

Figure 1

Downregulation of miR-483 in HCC. (A, upper and lower panels): Expression of miR-483-5p and miR-483-3p as reported in The Cancer Genome Atlas Liver Hepatocellular Carcinoma (TCGA-LIHC) data set is presented (MIR-TV: http://mirtv.ibms.sinica.edu.tw/analysis.php, accessed on 9 August 2022). p = 0.0001 compared with normal liver tissues. (B) Expression of mir-483-5p, miR-483-3p, and Notch 3 in HCC patient tumors of African American (AA), European American (EA), and Asian American (AAM) was analyzed by RNAseq. p values are presented in the graphs compared with normal HCC tissues (Upper, middle, and lower panels). (C) Endogenous expression of miR-483-5p and Notch 3 in HepaRG and HCC cells was determined by RT/qPCR. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to HepaRG cells. ns: not significant. (D) Cell lysates of HepaRG, HepG2, and SK-Hep1 cells were immunoblotted with anti-Notch 3, anti-Notch 2, anti-Notch 1, Hes1, and β-actin antibodies. The uncropped blots are shown in Supplementary Materials.