Table 1.
Functions, hydropathicity, name of cleaving enzymes/chemicals and properties of each amino acids of HNG peptide.
| Pos. | Amino Acid | Function | Hydropathicity | Amino Acid Side Chain Properties | Name of Cleaving Enzymes/Chemicals (Theoretical) |
|---|---|---|---|---|---|
| 1 | M | Neuroprotection | 1.900 | Hydrophobic | Chymotrypsin, Cyanogen Bromide |
| 2 | A | Neuroprotection | 1.800 | Hydrophobic | Proteinase K |
| 3 | P* | Neuroprotection | −1.600 | Hydrophobic | N/A |
| 4 | R* | Neuroprotection | −4.500 | Positive Charged (Basic) | Trypsin, Arg-C proteinase, Clostridiopeptidase B |
| 5 | G* | N/D | −0.400 | Hydrophobic | Pepsin, Thermolysin |
| 6 | F* | IGFBP-3 binding, Beta-Amyloid Binding | 2.800 | Hydrophobic | Chymotrypsin, Proteinase K |
| 7 | S* | Beta-Amyloid Binding, Beta-Amyloid Protection, Dimerization | −0.800 | Polar | 2-nitro-5-thiocyanobenzoic acid |
| 8 | C* | Neuroprotection, BAX, BAD, end tBID binding, Disulfide Bond for dimerization | 2.500 | Polar | Pepsin, Thermolysin |
| 9 | L* | Neuroprotection, Secretion, Dimerization | 3.800 | Hydrophobic | Proteinase K, Pepsin, Thermolysin, Chymotrypsin |
| 10 | L* | Secretion | 3.800 | Hydrophobic | Proteinase K, Pepsin, Thermolysin, Chymotrypsin |
| 11 | L* | Secretion | 3.800 | Hydrophobic | Proteinase K, Pepsin, Thermolysin, Chymotrypsin |
| 12 | L* | Neuroprotection | 3.800 | Hydrophobic | Proteinase K, Pepsin, Chymotrypsin |
| 13 | T* | Neuroprotection | −0.700 | Polar | Proteinase K, |
| 14 | G* | Neuroprotection | −0.400 | Hydrophobic | Asp-N Endopeptidase |
| 15 | E* | N/D | −3.500 | Negative Charged (Acidic) | Proteinase K, Glutamyl endopeptidase, Staphylococcal peptidase I |
| 16 | I* | N/D | 4.500 | Hydrophobic | Proteinase K, Asp-N Endopeptidase |
| 17 | D* | N/D | −3.500 | Negative Charged (Acidic) | Formic acid |
| 18 | L* | N/D | 3.800 | Hydrophobic | Proteinase K, Pepsin |
| 19 | P* | Beta-Amyloid Protection, Secretion | −1.600 | Hydrophobic | Thermolysin |
| 20 | V* | Secretion | 4.200 | Hydrophobic | Proteinase K, Peptidyl-Lys metalloendopeptidase |
| 21 | K | IGFBP-3 binding | −3.900 | Positive Charged (Basic) | Lysyl endopeptidase, Trypsin |
| 22 | R | N/D | −4.500 | Positive Charged (Basic) | Arg-C proteinase, Clostridiopeptidase B, Trypsin |
| 23 | R | N/D | −4.500 | Positive Charged (Basic) | Thermolysin, Arg-C proteinase, Clostridiopeptidase B, Trypsin |
| 24 | A | N/D | 1.800 | Hydrophobic | N/A |
* Neuroprotection core domains are shown in bold font. Pos.; Position, N/D; Not Determined, N/A: Not Available.