A. Illustration of in vivo experimental treatment set up.
B–D. Western blots showing pERK, total ERK, SNAI2, and BIM protein expression in two representative control tumors and trametinib-treated RD (B), SJRHB013_X (C), and SJRHB000026_X1 (D) tumors.
E–H. Relative tumor volume (RTV) of RD tumors receiving control (E), trametinib (F), IR (G), or combination trametinib + IR (H) treatment. Red line depicts 0.5 RTV; vertical dashed line represents the average EFS for each treatment arm. PD: progressive disease, PR: partial response.
I–L. Relative tumor volume (RTV) of SJRHB013_X tumors receiving control (I), trametinib (J), IR (K), or combination trametinib + IR (L) treatment. Red line depicts 0.5 RTV; vertical dashed line represents the average EFS for each treatment arm. PD: progressive disease, PR: partial response. CR: complete response. M–P. Relative tumor volume (RTV) of SJRHB000026_X1 tumors receiving control (M), trametinib (N), IR (O), or combination trametinib + IR (P) treatment. Red line depicts 0.5 RTV; vertical dashed line represents the average EFS for each treatment arm. PD: progressive disease, PR: partial response. CR: complete response.