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. 2023 Mar 21;12(6):954. doi: 10.3390/cells12060954

Figure 8.

Figure 8

Effect of antimycin A and 2-deoxy-D-glucose on the viability of cytokine-stimulated TSPO-deficient and control C20 microglia cells. (A) The effect of antimycin A (1–10 µM) or (B) 2-deoxy-D-glucose (5–300 mM) on cell viability was tested using the resazurin assay with TSPO-deficient and control C20 cells. Cells were treated with the cytokine cocktail TNFα, IL-1β, and INFγ (each of 1 ng/mL) for 40 h. Values represent survival normalized to conditions without antimycin A or 2-deoxy-D-glucose. Treating the cells with 300 mM 2-deoxy-D-glucose revealed a significantly different viability of TSPO−/− and control cells (p = 0.0026, t = 3991, df = 10, and unpaired t-test). For means and p values, see supplementary Tables S1 and S2. Stars indicate significance.