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Experimental design. Male and female young and aged C57BL/6J wild-type mice along with cSesn2−/− and Sesn2f/f C57BL/6J mice underwent a laparotomy and the clamping of the renal artery and vein for 15 min, followed by 24 h of reperfusion. Echocardiograms were performed, and tissues were collected for immunoblotting, histopathological, AKI induction, and mitochondrial respiration analyses.
