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. 2023 Mar 8;12(6):835. doi: 10.3390/cells12060835

Figure 2.

Figure 2

SCD5 expression in ccRCC cells is VHL dependent. (a) mRNA levels of VHL in HeLa cells infected with inducible control-shRNA or VHL-specific shRNA assayed by RT-qPCR; HSPCB was used as the reference gene. n = four. Data are presented as mean ± SEM. *** p < 0.001 (t-test). (b) Representative blot showing VHL expression in HeLa cells infected with lenti-control shRNA or lenti-VHL shRNA. Full-length blots are presented in Supplementary Figure S2. (c) SCD5 mRNA levels are affected by VHL. qPCR analysis of HeLa cells expressing shRNA against control or VHL. Two different primer sets were used for SCD5 mRNA analysis; HSPCB was used as the reference gene. n = four for each experiment. Data are mean ± SEM. *** p < 0.001 (t-test). (d) Analysis of SCD5 mRNA levels in RCC4 cells and RCC4 cells expressing VHL. Cell lysates were prepared from VHL-deficient RCC4 cells and cell lines transduced with VHL (RCC4 + VHL); GAPDH was used as the reference. n = four for each experiment. Data are mean ± SEM. *** p < 0.001 (t-test). (e) Western blot demonstrating SCD5 expression in RCC4 cells (VHL deficient) and RCC4 cells transduced with VHL. Full-length blots are presented in Supplementary Figure S2. Right panel shows quantification of relative SCD5 levels from three independent experiments. * p < 0.05 (t-test).