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. 2023 Mar 18;13(6):1166. doi: 10.3390/diagnostics13061166

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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SHP2^D61G/+ constitutively active mutant negatively regulates fibroblast migration, myofibroblast differentiation, and proliferation. (A,B) SHP2^D61G/+ constitutively active mutant lung fibroblasts exhibited significantly reduced cell migration at all time points (T0-T24, 2-fold) compared to wild-type ones (SHP2^+/+). One way ANOVA, * p < 0.05. (C) Double immunofluorescence analysis in representative primary MLF samples showing decrease in TGFB1-induced a-SMA (red) and stress fibers as indicated by phalloidin green (merged-yellow) in SHP2^D61G/+ constitutively active mutant lung fibroblasts compared to wild-type ones (SHP2^+/+). (D) PDGF-BB stimulation induced a significant increase (1.4-fold) in proliferation rates of SHP2^+/+ MLFs compared to the SHP2^D61G/+. Each bar represents mean expression of 6 samples (biological replicates). Data (absorbance) represent mean + SD. One-way ANOVA, * p < 0.05. Details for this figure are presented in Supplemental Data S1.