Skip to main content
. 2023 Mar 10;12(6):863. doi: 10.3390/cells12060863

Figure 2.

Figure 2

Human B3GnT2 promotes Wnt/β-catenin signalling and acts at the level of LRP6. (a,b) TOPFLASH Wnt reporter assays (upper graphs) and Western blots (lower blots) showing that human B3GnT2, but not B3GnT8, modifies LRP6 and promotes Wnt/β-catenin signalling in a dose-dependent manner. Amounts transfected: LRP6, 20 ng; hB3GnT2 or hB3GnT8, as indicated. Data represent mean + SD. **** p < 0.0001. n.s., not significant. (c) TOPFLASH reporter assay in HEK293T cells in 96-well format. Amounts transfected: LRP6, 20 ng; β-catenin, 50 ng; hB3GnT2, 0.5 ng. Data represent mean + SD. ** p < 0.01 (d) Western blots of cell lysates from HEK293T cells in 96-well format. Amounts transfected: hLRP6, 20 ng; mWnt3a, 8 ng; V5-mFz5 or V5-mFz8, 20 ng; hB3GnT2, as indicated.