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. 2023 Mar 28;18(3):e0282298. doi: 10.1371/journal.pone.0282298

Fig 2. The optical sectioning capabilities of LSM permits visualization into the hydrogel microcarrier.

Fig 2

a) 2D max. intensity and orthogonal projection of P7 D3 microcarrier with a single CTG-expressing cell. 2D max. intensity projections using the middle 1/3 of the volume to view the interior of the microcarrier using b) DRAQ-5, c) CTG, d) DiI plasma membrane stain, and e) elastic scattering contrast. Owing to the optical sectioning capabilities of LSM and refractive index matching of the microcarriers, biological features such as cell infiltration into the microcarrier can be visualized (white arrow). Scale bar = 25 μm.