Figure 4.

Cervical cancer cells express higher phosphorylation levels of PI3K/Akt1 than ERK1/2 in response to insulin treatment. (A) PI3K, phospho PI3K. (B) Akt1, phospho Akt1. (C) ERK1/2 and phospho ERK1/2 protein levels were analyzed by WB in whole-cell lysates from HeLa cells stimulated with 50 nM insulin. Data are representative of 3 independent experiments. Graphics show densitometric analysis of total levels and phosphorylated protein. The first bar is the control group without treatment. All experiments were performed in three independent experiments in triplicate, and experimental data were expressed as mean ± standard deviation (SD). * p < 0.05 and ** p < 0.01 compared to control (taken as 100%).