Figure 5.

Insulin treatment increases migration of HeLa cells. HeLa cells were stimulated with different concentrations of insulin in the presence of mitomycin C and with DMEM medium without SFB; cells were treated with mitomycin C in the control group. Cell migration was evaluated by quantifying the reduction in the open area (a lower percentage of the open area means a higher percentage of cell migration). The histograms represent the mean value ± standard error of the mean (SEM) of the percentage of the open area of three independent experiments in triplicate. One-way ANOVA was performed, followed by the Tukey post hoc test to compare the treated groups against the control group. * p < 0.05; ** p < 0.01.