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. 2023 Mar 9;45(3):2296–2308. doi: 10.3390/cimb45030148

Table 1.

Oligonucleotides used for PCR.

Target Primer Sequence 5′-3′ Position Size (bp)
IR 5′-AACCAGAGTGAGTATGAGGAT-3′ nt 2201-2221 Isoform B: 636
5′-CCGTTCCAGAGCGAAGTGCTT-3′ nt 2780-2800 Isoform A: 600
IRS1 5′-TCCACTGTGACACCAGAATAAT-3 nt 4011-4032 763
5′-CGCCAACATTGTTCATTCCAA-3′ nt 4753-4773
IRS2 5′-TAGGCATCAATGGGTGGTATTT-3′ nt 6358-6380 116
5′-CTACGGATAGAGGGCGAGTTA-3′ nt 6453-6474
GAPDH 5′-ACCACAGTCCATGCCATCAC-3′ nt 602-621 451
5′-TCCACCACCCTGTTGCTGTA-3′ nt 1031-1053