Table 2.
List of primary and secondary antibodies.
| Primary Antibody | Epitope/ Specificity |
Host Species | Type | Dilution | Source |
|---|---|---|---|---|---|
| Insulin Receptor β (C19) | C-terminus/IgG | Rabbit | Polyclonal | 1:500 | Santa Cruz SC-711 |
| IRS1 (C-20) | C-terminus/IgG | Rabbit | Polyclonal | 1:500 | Santa Cruz SC-559 |
| IRS2 (H-205) | aa 926-1130/IgG | Rabbit | Polyclonal | 1:500 | Santa Cruz SC-8299 |
| PI 3-kinase (Z-8) | p85α/IgG | Rabbit | Polyclonal | 1:500 | Santa Cruz SC-423 |
| ERK 1 (C-16) | C-terminus/IgG | Rabbit | Polyclonal | 1:500 | Santa Cruz SC-93 |
| Akt1 (B1) | aa 345-480/IgG1 | Mouse | Monoclonal | 1:500 | Santa Cruz SC-5298 |
| Phospho insulin receptor | Phospho Tyr/1361/IgG | Rabbit | Polyclonal | 1:500 | Abcam ab60946 |
| Phospho-IRS1 | Phospho Tyr/632/ IgG |
Rabbit | Monoclonal | 1:500 | Abcam ab109543 |
| Anti- phospho Tyrosine | Tyrosine-phosphorylated proteins/IgG2b | Mouse | Monoclonal | 1:500 | Millipore 05-947 |
| Phospho-Akt1 | Phospho Thr 308/IgG | Rabbit | Monoclonal | 1:500 | Millipore 05-802R |
| Phospho-Erk1/2 | Phospho Thr 202/Tyr 204, Thr 185/Tyr 187/IgG | Rabbit | Monoclonal | 1:500 | Millipore 05-797R |
| Phospho-PI3K p85 | p85 Phospho Tyr 467, Tyr 199/IgG | Rabbit | Polyclonal | 1:500 | GeneTex GTX132597 |
| β-actin (4E8H3) | IgG1 | Mouse | Monoclonal | 1:500 | [29] |
| Secondary antibody |
Epitope
Specificity |
Host species | Type | Dilution | Source |
| Rabbit anti-goat IgG HRP-coupled | Rabbit | Polyclonal | 1:5000 | Santa Cruz SC-2768 | |
| Mouse IgG HRP-coupled | Mouse | Polyclonal | 1:5000 | GeneTex GTX213111-01 |