Table 2.
Detections of low viral and Mp loads with R-GENE® PCR assays, QS and FA.
| Target | Dilution | Detected by R-GENE® | R-GENE® Median Ct Value | R-GENE® Median Titer (copies/mL) | Detected by FA |
Detected by QS |
QS Ct Value |
|---|---|---|---|---|---|---|---|
| EV-A71-C1 | 1 | 5/5 | 34.99 | ND | ND | 1/1 | 36.0 |
| 1/10 | 5/5 | 37.7 | ND | 1/1 | 3/6 | 38.5/38.7/38.5 | |
| 1/100 | 3/5 | 38.22 | ND | ND | ND | ND | |
| EV-D68-B3 | 1 | 5/5 | 32.71 | ND | 1/1 | 0/1 | ND |
| 1/10 | 5/5 | 35.57 | ND | 5/6 | 0/1 | ND | |
| 1/100 | 4/5 | 40 | ND | ND | ND | ND | |
| E-30 | 1 | 5/5 | 34.52 | ND | ND | 1/1 | 37.5 |
| 1/10 | 5/5 | 37.49 | ND | 1/1 | 0/6 | ND | |
| 1/100 | 3/5 | 40 | ND | ND | ND | ND | |
| E-6 | 1/10 | 5/5 | 38.58 | ND | 1/1 | 1/1 | 38.5 |
| 1/100 | 3/5 | 40 | ND | ND | ND | ND | |
| PeV-1 | 1/10 | 5/5 | 38.41 | ND | 1/1 | 1/1 | 33.4 |
| 1/100 | 3/5 | 40 | ND | ND | ND | ND | |
| PeV-3 | 1/10 | 5/5 | 37.52 | ND | 1/1 | 1/1 | 35.0 |
| 1/100 | 3/5 | 40 | ND | ND | ND | ND | |
| HSV-1 | 1/10 | 5/5 | 36.41 | 1280 | 1/1 | 1/1 | 35.4 |
| 1/100 | 2/5 | 39.03 | <250 | ND | ND | ND | |
| HSV-2 | 1/10 | 5/5 | 35.39 | 277 | 1/1 | 1/1 | 36.5 |
| 1/100 | 3/5 | 37.61 | <100 | ND | ND | ND | |
| HHV-6 | 1/10 | 5/5 | 35.83 | 534 | 1/1 | 1/1 | 37.2 |
| 1/100 | 1/5 | 39.28 | <200 | ND | ND | ND | |
| VZV | 1/10 | 5/5 | 37.25 | <300 | 1/1 | 1/1 | 35.6 |
| 1/100 | 3/5 | 40 | <300 | ND | ND | ND | |
| Mp | 1/10 | 5/5 | 36.21 | ND | ND | 1/1 | 34.8 |
| 1/100 | 2/5 | 39.32 | ND | ND | ND | ND |
Ct: cycle threshold; EV: Enterovirus; E: Echovirus; PeV: Parechovirus; HSV: Herpes simplex virus; HHV-6: Human herpesvirus 6; VZV: Varicella-zoster virus; Mp: Mycoplasma pneumoniae. ND: Not determined.