Figure 6.

(A) Process of Agrobacterium tumefaciens mediated transformation of S. lycopersium var. Pusa Ruby and Pusa Early Dwarf. The image here represents Pusa Ruby only. (B) Explants not treated by Agrobacterium culture treated as control. (C) T1 transgenic plants growing in a greenhouse facility. (E) PCR and (D) kanamycin assay was used to select tranformants. Primers specific for kanamycin gene (nptII) were used for PCR that resulted in 599 bp long fragment. The PCR shown here is for one batch of plants with first two wells (1 and 2 in stack 1) with negative control wild type plants and 1 kb+ DNA ladder by Fisher scientific. (F) Regeneration and transformation efficiencies of Pusa Ruby (PR) and Pusa Early Dwarf (PED).