. 2023 Mar 11;12(6):1188. doi: 10.3390/foods12061188

Table 2.

Detailed parameters of the extraction processes.

Superclean^TM Extraction Tubes Properties
Tube type		Envi-8	Envi-18		LC-8	LC-18	LC-CN	LC-Ph
Bed type		C₈ (octyl)	C₁₈ (octadecyl)		C₈ (octyl)	C₁₈ (octadecyl)	Cyano	Phenyl
Bed mass (mg)		1000	1000		500	1000	500	500
Tube volume (mL)		6	6		6	6	6	3
Carbon Loading (%)		14	17		7	11.5	7	5.5
Solid Phase Extraction Steps
Conditioning	solvents	5 mL MeOH	5.0 mL ACN 5.0 mL MeOH	3.0 mL DCM 3.0 mL ACN 3.0 mL MeOH	5.0 mL ACN 5.0 mL MeOH	5.0 mL ACN 5.0 mL MeOH	5.0 mL ACN 5.0 mL MeOH	5.0 mL ACN 5.0 mL MeOH
Conditioning	velocity	10 mL/min
Bed washing	matrix	5.0 mL deionized water
Bed washing	velocity	10 mL/min
Sample flow	volume	100 mL
Sample flow	velocity	1 mL/min
Drying		5 min under vacuum
Elution	solvents	3 mL MeOH	1.5 mL ACN 1.5 mL MeOH	2.0 mL DCM 1.5 mL ACN 1.5 mL MeOH	1.5 mL ACN 1.5 mL MeOH	1.5 mL ACN 1.5 mL MeOH	1.5 mL ACN 1.5 mL MeOH	1.5 mL ACN 1.5 mL MeOH
Elution	velocity	10 mL/min