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. 2023 Mar 4;14(3):651. doi: 10.3390/genes14030651

Figure 4.

Figure 4

ALDH1A3 was screened and identified as the downstream target gene of EGFRvIII. (A) Differential gene expression analysis of LN229-EGFRvIII (+) cells and U87-EGFRvIII (+) cells. (B) Analysis of common differential gene expression of U87-EGFRvIII (+) cells and LN229-EGFRvIII (+) cells. (C) The mRNA expression of ALDH1A3 was detected by qRT-PCR in U87-EGFRvIII (+) cells, LN229-EGFRvIII (+) cells, and BNI-21-1-S-EGFRvIII (+) cells. (D) The protein expression of ALDH1A3 was detected by Western blot. (E) The expression of EGFRvIII and ALDH1A3 was detected by immunohistochemistry in tumor tissue from the orthotopic xenograft tumor model of mice (scale bars: 100 μm) (n = 3 per group). Representative data are determined from three independent experiments (n = 3 per group). vs. EGFRvIII (−). ** p <0.01, **** p <0.0001 by the two-tailed Student’s t-test.