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Orthogonal experimental factor level table of dye addition and dyeing time.
| Level | Amount of CFDA Added (μL) | Dyeing Time t1 (s) | Amount of PI Added (μL) | Dyeing Time t2 (s) |
|---|---|---|---|---|
| 1 | 5 | 5 | 5 | 5 |
| 2 | 10 | 10 | 10 | 10 |
| 3 | 15 | 15 | 15 | 15 |
Remarks: CFDA = 5(6)-carboxyfluorescein diacetate succinimide ester; PI = propidium iodide.
