Table 3.
Orthogonal test for identifying the optimal combination of dye concentration and dyeing time.
| Experiment | Parameters | Viable Count (AFU/μL) | |||
|---|---|---|---|---|---|
| A CFDA Amount (μL) |
B Dyeing Time t1 (s) |
C PI Amount (μL) |
D Dyeing Time t2 (s) |
||
| 1 | 5 | 5 | 5 | 5 | 1661 |
| 2 | 5 | 10 | 10 | 15 | 1762 |
| 3 | 5 | 15 | 15 | 10 | 1802 |
| 4 | 10 | 15 | 5 | 15 | 1897 |
| 5 | 10 | 5 | 10 | 10 | 1782 |
| 6 | 10 | 10 | 15 | 5 | 1739 |
| 7 | 15 | 5 | 15 | 15 | 1628 |
| 8 | 15 | 15 | 10 | 5 | 1786 |
| 9 | 15 | 10 | 5 | 10 | 1824 |
| K1 | 1742 | 1691 | 1794 | 1729 | |
| K2 | 1806 | 1775 | 1777 | 1803 | |
| K3 | 1746 | 1829 | 1723 | 1763 | |
| R | 64 | 138 | 71 | 74 | |
| Factor ranking | B > D > C > A | ||||
| Optimal combination | A2B3C1D2 | ||||