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. 2023 Mar 18;9(3):243. doi: 10.3390/gels9030243

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The effect of Cripto produced in 3D microcarriers on C2C12 cell proliferation detected by the BrdU incorporation assay. (A) C2C12 myoblast proliferation was evaluated by the BrdU incorporation assay after being cultured for 48 h in serum-free medium containing Cripto produced in 3D microcarriers (Cripto_(3D)) or commercially available Cripto (Cripto_(R&D)). Also evaluated were a bFGF medium positive control and a serum-free medium negative control. (B) The recombinant Cripto induces myoblast proliferation in a dose-dependent pattern; increasing concentrations of Cripto_(3D) and Cripto_(R&D) were added to C2C12 cells, and proliferation was quantified by the BrdU incorporation assay. (C) Cell proliferation was further evaluated by counting the total number of live cells. The proliferative effect of Cripto_(3D) was compared with that of commercial Cripto_(R&D). The data are presented as mean ± S.D. from at least three independent experiments. * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001.