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. 2023 Mar 11;24(6):5372. doi: 10.3390/ijms24065372

Figure 9.

Figure 9

Validation of three predicted RNA editing sites (rpl5-509, rpl5-512, and rpl5-529) using PCR and Sanger sequencing. The top panel shows the sequences and chromatographs from the PCR products amplified using gDNA as the template. The bottom panel shows the sequences and chromatographs from the PCR products amplified using the cDNA. The gene names and the RNA editing sites’ positions are shown at the top of the figure, separated with “-”. The red squares highlight the focal RNA editing site. rpl5-529 created a new stop codon upstream from the stop codon found in the genomic sequence.