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. 2023 Mar 12;24(6):5419. doi: 10.3390/ijms24065419

Figure 4.

Figure 4

MPE and MSE reduce lipid accumulation, preventing lipogenesis and promoting lipolysis. Differentiated 3T3-L1 adipocytes were treated with 500 µM PA for 48 h in the presence or absence of 100 µg/mL MPE or MSE, as reported in Section 4. Then, cell lysates were analyzed by Western blotting using specific primary antibodies directed against PPARγ, Perilipin-2, phosphorylated AMPK (p-AMPK), phosphorylated ACC (p-ACC) and phosphorylated HSL (p-HSL). Equal amounts of proteins were loaded in each lane (30 μg) as normalized by γ-Tubulin detection. The bar graphs represent the means of three independent experiments ±SD. The statistical differences between groups were evaluated using a one-way ANOVA test. * p < 0.05, ** p < 0.01 were significant with respect to differentiated 3T3-L1 adipocytes. # p < 0.05, ## p < 0.01, ### p < 0.001 were significant with respect to PA-treated differentiated 3T3-L1 adipocytes.