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. 2023 Mar 12;24(6):5419. doi: 10.3390/ijms24065419

Figure 5.

Figure 5

MPE and MSE reduce PA-induced ER stress in 3T3-L1 adipocytes. Differentiated 3T3-L1 adipocytes were treated with 500 µM PA for 48 h in the presence or absence of 100 µg/mL MPE or MSE. Cell lysates underwent Western blotting analysis for ER stress protein markers PERK, GRP78 and CHOP, as well as for phosphorylated JNK (p-JNK). Equal loading of protein (30 µg) was verified by immunoblotting for γ-Tubulin. The bar graphs represent the means of three independent experiments ± SD. The statistical differences between groups were evaluated using a one-way ANOVA test. * p < 0.05, ** p < 0.01 and *** p < 0.001 were significant with respect to differentiated 3T3-L1 adipocytes. ## p < 0.01, ### p < 0.001 were significant with respect to PA-treated differentiated 3T3-L1 adipocytes.