Figure 8.

MPE and MSE increase the expression levels of the anti-oxidant molecules. Differentiated 3T3-L1 adipocytes were treated with 500 µM PA for 48 h in the presence or absence of 100 µg/mL MPE or MSE. Cell lysates underwent Western blotting analysis for Nrf2, MnSOD and HO-1. An equal loading (30 µg) of proteins was verified by immunoblotting for γ-Tubulin. The bar graphs represent the means of three independent experiments ±SD. The statistical differences between groups were evaluated using a one-way ANOVA test. * p < 0.05, ** p < 0.01 and *** p < 0.001 were significant with respect to differentiated 3T3-L1 adipocytes. ## p < 0.01 was significant with respect to PA-treated differentiated 3T3-L1 adipocytes.