. 2023 Mar 7;24(6):5088. doi: 10.3390/ijms24065088

Table 8.

Toxicity profiling of different oxygen substituted anilines based on the scaffold of analog 72 (101–112) ^a,b,c.


Name	X	R¹	R²	R³	R⁴	CM	CMI	ROS	GSH	NMI1	CC	NMI2	MMP	CHI
Erlotinib	-					0.77	0.50	0.13	0.63	0.10	0.10	0.062	0.12	0.40
72	CH	H	OMe	OMe	OMe	0.89	0.78	0.063	0.096	0.24	0.50	0.064	0.078	0.37
101	CH	H	OMe	H	OMe	0.96	0.77	0.69	0.64	0.78	0.88	0.65	0.84	0.59
102	CH	H	OMe	OMe	H	0.72	0.63	0.21	0.059	0.43	0.35	0.30	0.24	0.42
103	CH	OMe	H	OMe	H	0.091	0.24	0.030	0.12	0.65	0.40	0.034	0.032	0.26
104	CH	OMe	H	H	OMe	1.00	1.00	0.99	0.98	0.91	0.99	0.97	0.93	0.79
105	CH	H	H	OMe	H	0.0067	0.18	0.036	0.16	0.69	0.61	0.050	0.11	0.30
106	CH	H	OMe	H	H	0.69	0.25	0.089	0.24	0.085	0.90	0.27	0.62	0.37
107	CH	OMe	H	H	H	1.00	1.00	1.00	1.00	0.94	0.98	0.97	0.99	0.81
108	CH	H	OCH₂O		H	0.98	1.00	1.00	0.99	0.87	0.98	0.96	0.98	0.69
109	CH	H	OCH₂CH₂O		H	0.37	0.90	0.44	0.43	0.40	0.79	0.19	0.49	0.47
110	N	H	OMe	OMe	OMe	0.81	0.43	0.065	0.38	0.75	0.80	0.47	0.47	0.45
111	N	H	OMe	H	OMe	0.013	0.062	0.063	0.018	0.37	0.41	0.026	0.020	0.25
112	N	H	H	OMe	H	0.0021	0.0032	0.19	0.0067	0.37	0.24	0.003	0.023	0.18

^a Abbreviations from columns left to right. CM: Cell morphology; CMI: Cell membrane integrity; ROS: Reactive oxygen species; GSH: Glutathione; NMI1: Nuclear membrane integrity 1; CC: Cell Cycle; NMI2: Nuclear membrane integrity 2; MMP: Mitochondrial membrane potential; CHI: Cell Health Index. ^b Traffic light colouring matching classifier values (Red–Green: high toxicity risk–low toxicity risk). ^c all n = 2.