Figure 1.

Four groups’ experimental protocols. (A) Perfusion series 1: liver subjected to 30 min warm ischemia in situ, flush, and static cold storage; and HMP or HOPE after static cold storage (n = 6 each). Perfusion series 2: livers except WI 30 min were perfused for 2 h using NMP to determine liver function and injury (n = 3 each). (B) Liver appearance before warm ischemia for 30 min and flush. (C) Liver morphology in three different hyperthymic storage or machine perfusion methods in perfusion series 1. (D) The machine system stored the livers and performed machine perfusion. (E) Schematic representation of SCS and MP devices. (F) The parameters of pH, PO₂, and PCO₂ of hyperthymic machine perfusion were calculated.