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. 2023 Mar 11;24(6):5403. doi: 10.3390/ijms24065403

Figure 4.

Figure 4

Nuclear damage and apoptosis induced by hypoxia-ischemic injury. (A) Enzyme-linked immunosorbent assay (ELISA) kit of 8-OHdG and Cyt C in liver tissue. (B) IHC staining of HMGB1 was performed on the paraffin-embedded section of rat liver tissues. Scale bar: 20 μm; (C) IHC-stained HMGB1 were quantified using Image J. (D) Apoptotic cells were evaluated using the TUNEL assay, and the number of apoptotic cells was counted. Scale bar: 250 μm; (E) Western blot shows the apoptosis-related protein level in four different groups; (F) TUNEL-positive cells and quantitative analysis of the relative intensity of Cleaved-Caspase 3, Bax, and Bcl-2 normalized to the GAPDH loading control were quantified using Image J (n = 6 per group for (A); n = 3 independent experiments for (B–E)). Mean ± SD, N.S., p > 0.05, * p < 0.05.