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. 2023 Mar 7;24(6):5096. doi: 10.3390/ijms24065096

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Gene map of recombinant plasmids with inserted P. phosphoreum lux genes. Recombinant pRFN4 plasmids used in this study: pRFN4, pRFN4-Pp N-lumP, pRFN4-Pp luxCP, pRFN4-Pp luxCP-N-lumP, and pRFN4-Pp luxLP-N-lumP plasmids. The nomenclature of riboflavin biosynthetic genes of B. subtilis differs from other species. Therefore, ribE in pRF2 and pRFN4 is the gene coding for riboflavin synthase in B. subtilis [5]. In pRFN4, the ribE gene coding for Phe 2 in riboflavin synthase was replaced with Ala for overproduction of lumazine (F2A in ribB) [13].