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. 2023 Mar 7;24(6):5096. doi: 10.3390/ijms24065096

Figure 9.

Figure 9

Relative fluorescence intensities. 1, E. coli 43R itself; 2, pRFN4 in E. coli 43R; 3, pRFN4-Pp N-lumP in E. coli 43R, 4, pRFN4-Pp luxLP-N-lumP in E. coli 43R; 5, pRFN4-Pp luxCP-N-lumP in E. coli 43R; 6, pRFN4-Pp luxCP in E. coli 43R. (a) Excitation wavelength was fixed at 410 nm for lumazine. (b) Excitation wavelength was fixed at 450 nm for riboflavin. (c) Identification of lumazine and riboflavin in TLC (thin layer chromatography) panel under UV illumination. 1, Mixtures of lumazine and riboflavin; 2, cell free extract of pRFN4 in E. coli 43R; 3, cell free extract of pRFN4-Pp luxLP-N-lumP in E. coli 43R. Lum, lumazine; Rf, riboflavin.