Table 1.
Strains | Characteristics | Source |
E. coli XL-1 Blue | Cloning strain | Real Biotech Corporation |
E. coli 43R | Mutant of E. coli RR1 strain | Miyamoto.et al. (1987) J. Bacteriol. [16] |
Plasmids | Characteristics | Source |
pRFN4 | Recombinant plasmid containing the riboflavin genes from Bacillus subtilis | Illarionov et al. (2004) J. Org. Chem. [13] |
pT7-5 PpEE | Recombinant pT7-5 plasmid containing the luxL and luxC genes, as well as their intergenic DNA from P. phosphoreum NCBM 844 | Woo et al. (2005) Kor. J. Microbiol. [14] |
pRFN4- Pp N-lumP |
pRFN4 plasmid inserting the gene of 5′-terminal half of luxL for N-LumP from P. phosphoreum | This study |
pRFN4- Pp luxCP |
pRFN4 plasmid inserting the promoter region for luxC from P. phosphoreum | This study |
pRFN4- Pp luxCP-N-lumP |
pRFN4-Pp N-lumP plasmid inserting the promoter region for luxC from P. phosphoreum | This study |
pRFN4- Pp luxLP-N-lumP |
pRFN4-Pp N-lumP plasmid inserting the DNA for promoter region for luxL from P. phosphoreum | This study |