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. 2023 Mar 7;24(6):5093. doi: 10.3390/ijms24065093

Figure 4.

Figure 4

Uridine supplementation alleviated oxidative stress and lipid peroxidation in THP-1 cells. (A) Relative UPP1 mRNA expression levels after LPS (10 μg/mL) stimulation 8 h in THP-1, HUVEC and A549 cells. (B–D) Relative mRNA expression levels of IL1β, IL6 and TNFα in THP-1 cells after LPS stimulation with uridine supplementation in different concentration. LPS and uridine were almost simultaneously administrated. (E) Representative images of fluorescence probe for ROS in the indicated group in THP-1 cells after LPS stimulation with or without uridine (200 μM) supplementation. (F) Representative images of fluorescence probe for lipid peroxidation in the indicated group in THP-1 cells. (G) The statistical results for ROS. (H) The statistical results for lipid peroxidation. Green and red fluorescence represents oxidative and reduced lipid respectively. (I,J) Relative GSH and MDA level in THP-1 cells lysates. n = 6 per group, (Data are presented as Mean ± SD, *** p < 0.001 compared with indicated group, ns, no significance).