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. 2023 Mar 7;24(6):5093. doi: 10.3390/ijms24065093

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Nrf2 knockdown via siRNA abrogated the antioxidative effect of uridine. (A) Western blots for Nrf2, HO1, SLC7A11 and GPX4 protein of THP-1 cells in the indicated group. (B,C) Relative GSH and MDA levels in the indicated group. NC means negative control. (D) Representative images of fluorescence probe for mitochondrial membrane potential determined by JC-1. n = 6 per group. (Data are presented as Mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001 compared with indicated group, ns, no significance).