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. 2023 Mar 7;24(6):5082. doi: 10.3390/ijms24065082

Figure 2.

Figure 2

OxLDL/LOX-1 induces the activation of NF-κB in CRPC cell models. Western blot analysis of p-p65 and p-IκB-α in protein extracts of (A) C4-2B and (C) 22Rv1 cells transfected with siRNA against LOX-1 or control and treated with 50 μg/mL oxLDLs for 1 h. The NF-κB activity was analyzed using the reporter plasmid pHAGE NFKB-TA-LUC-UBC-dTomato-W in (B) C4-2B or (D) 22RV1 cells, co-transfected with siRNA control or siRNA LOX-1 and treated with 50 μg/mL oxLDLs for 24 h. The data represent the means ± S.D. of three independent experiments analyzed by one-way analysis of variance and Dunnett’s post-test (**** p ≤ 0.0001, *** p ≤ 0.001, ** p ≤ 0.01, * p ≤ 0.05, ns: non-significant statistical difference).