Table 2. Structural parameters of microtubules assembled in the presence of different nucleotides and drugs*.
Paclitaxel-pre microtubules | Paclitaxel- post microtubules | Paclitaxel- GDP tubulin | GDP-microtubules | GMPCPP-microtubules | Docetaxel-microtubules | Baccatin III-microtubules | 2a-microtubules | 2b-microtubules | |
---|---|---|---|---|---|---|---|---|---|
Microtubule radius (nm) | 10.97±0.10 | 11.04±0.51 | 10.98±0.47 | 11.42±0.10 | 11.63±0.10 | 11.53±0.10 | 11.06±0.35 | 11.27±0.57 | 11.60±0.36 |
Avg. PF number | 12.21±0.10 | 12.28±0.71 | 12.23±0.65 | 12.91±0.10 | 13.29±0.10 | 12.90±0.10 | 12.29±0.39 | 12.63±0.72 | 12.99±0.40 |
Inter-PF distances (nm) | 5.58±0.01 | 5.59±0.33 | 5.57±0.29 | 5.50±0.03 | 5.45±0.03 | 5.57±0.01 | 5.61±0.18 | 5.55±0.31 | 5.56±0.17 |
Avg. monomer length (nm) | 4.18±0.01 | 4.18±0.01 | 4.18±0.01 | 4.06±0.01 | 4.18±0.010 | 4.18±0.01 | 4.16±0.03 | 4.15±0.03 | 4.13±0.03 |
1 nm band peak position (nm–1) | 6.02±0.01 | 6.02±0.01 | 6.02±0.01 | 6.19±0.01 | 6.02±0.01 | 6.02±0.01 | 6.04±0.5 | 6.06±0.05 | 6.08±0.05 |
8 nm band | Yes | Yes | Yes | No | Yes | Yes | Yes | Yes | Yes |
Errors are SE of three independent.