Table 3.

Early postnatal inflammation—study designs.

References	Species	Environmental Enrichment		LPS Injection(s)
	(Age and Timing of Evaluations)	Type of Enrichment	Subjects and Duration	(Time and Dose)
MacRae et al., 2015 [120]	Sprague–Dawley rats Dams: age not specified Pups: newborn (Evaluation of effects in male and female offspring (P 40–P 90))	Large multi-level cage with toys and tubes changed twice weekly, chew bone, Nestlets©, and ramps. Four to six animals per cage.	On dams: EE was conducted from breeding to weaning of pups. On pups: EE was conducted from birth to the end of the experiment (P 1–P 90).	On pups: P 3 and P 5, 2 i.p. injections of LPS (50 μg/kg). (LPS administered when EE protocol is already ongoing)
Wu et al., 2022 [121]	C57BL/6 mice. Pups: 10 days (Evaluation of effects in male offspring P 39–P 42)	Cage (60 × 32 × 38 cm) with running wheel, swing, platforms and toys. Objects were changed twice a week. Number of animals per cage not specified.	On pups: EE was conducted four hours per day during P 10–P 38.	On pups: P 10, i.p. injection of LPS (100 μg/kg). (LPS before EE protocol)
Pavlova et al., 2022 [122]	Wistar rats Pups: newborn (Evaluation of effects in male and female offspring P 90–P 105)	Plastic box (51 × 40.5 × 30 cm) containing stairs, a running wheel, tubes, and materials for burying (sawdust, foam filler, paper, etc.). Four to six animals per cage.	On pups: EE was conducted for 20 min every other day, from the age of 45 days until the end of experiment (~3.5 months).	On pups: P 3 and P 5, 2 i.p. injection of LPS (dose of 50 μg/kg in a volume of 10 μL/g). (LPS before EE protocol)

EE: environmental enrichment; i.p.: intraperitoneal; LPS: lipopolysaccharide; P: postnatal day.