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. 2023 Mar 9;24(6):5283. doi: 10.3390/ijms24065283

Figure 2.

Figure 2

The co-culture BBB model showed efficient barrier properties. Assays were used to analyze the barrier property of bEnd.3 and C8-D1A cells seeded individually or grown in co-culture conditions. (A) Transendothelial electrical resistance (TEER) analysis using chopstick electrodes to record resistance across the transwell membrane grown with bEnd.3, C8-D1A cells or co-cultured. (B) Fluorescein isothiocyanate (FITC) dextran permeability assay was used to analyze the barrier capacity of the in vitro BBB model. (C) Solvent persistence and (D) solvent leakage tests were used to measure how long bEnd.3, C8-D1A, and co-cultured cells can hold the media on the apical side of the transwell. All data had a sample size of three (n = 3) and three experimental repeats. Data are presented as mean ± SEM. The mono-culture models were compared to the co-culture models (* bEnd.3, # C8-D1A). p < 0.05 was considered to be statistically significant. *;# p < 0.05, **;## p ≤ 0.01 and ***;### p ≤ 0.001.