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. 2023 Mar 9;24(6):5283. doi: 10.3390/ijms24065283

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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PA-BSA affects the expression of tight junction proteins. (A) Confocal microscope images show the co-expression of ZO-1 in bEnd.3 cells in the control and the bovine serum albumin-conjugated palmitic acid (PA-BSA)-treated group. (B) Immunoblot analysis was used to compare the expression levels of tight junction proteins ZO-1, occludin-1, and claudin-5 expressed in bEnd.3 cells from the control and PA-BSA-treated group. (C) TEER resistance values were measured across the co-cultured transwell membrane of the control group and PA-BSA-treated group. All data had a sample size of three (n = 3) and three experimental repeats. Data are presented as mean ± SEM. p < 0.05 was considered to be statistically significant. ** p ≤ 0.01 and *** p ≤ 0.001.