Figure 4.

ONC-induced degradation of optic nerve myelination was partially prevented by synaptamide or A8 treatment. (A) Cross section of the optic nerve immunostained for MBP indicates the loss of the axon structure at 12 weeks after ONC, which is partially reversed by synaptamide or A8 injection. (B) The fluorescent intensities of MBP staining were quantified by setting the whole captured image as the region of interest. The signal intensity was shown relative to the sham control group. (C,D) Immunoblot of myelination marker proteins, MBP, CNPase and O2 from optic nerves collected at 12 weeks after ONC from sham and vehicle-, synaptamide- or A8-treated group (C), with quantitative results normalized by GAPDH from three mice per group (D). Data are expressed as mean ± s.e.m. (n = 3), representing two independent experiments. ** p < 0.01, *** p < 0.001. Scale bar, 10 μm.