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. 2023 Mar 9;24(6):5252. doi: 10.3390/ijms24065252

Figure 1.

Figure 1

Spheroids as a model of cancer stem cells (CSCs) enrichment of CRC cell lines. (A–G) Comparative expression of surface markers associated with stemness in second-generation spheroids compared to monolayer cultures (2D) in RKO, SW480, and SW620 cell lines. The proportions of CD44 (B), Lgr5 (C), CD44v6 (E), CD133 (F,G), CD44+Lgr5+, and CD44−Lgr5+ subpopulations (A,D) were measured by flow cytometry. (H) Quantification of the spheroid formation efficiency (SFE %) of the RKO, SW480, and SW620 cell lines throughout four consecutive generations. Data are represented as the mean values ± SEM of at least three independent experiments. (B–F) Student’s t-test; (G) One-way ANOVA followed by Bonferroni’s multiple comparisons. * p < 0.05; ** p < 0.001; *** p < 0.0001; **** p < 0.00001.