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. 2023 Mar 11;24(6):5393. doi: 10.3390/ijms24065393

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Phylogenetic analysis and multiple sequence alignment of C4Hs proteins (A) The phylogenetic tree containing C4H members from safflower and other plant species. The tree was constructed with MEGA 6.0 using the neighbor-joining (NJ) method and 1000 bootstrap replications. (B) Multiple sequence alignment of CtC4H1 and C4H from other plant species. The P450 conserved sequences are boxed in red, the underlined regions represent the six SRSs and Triangle represents E-R-R triad; Cb: Chrysanthemum boreale; Cs: Camellia sinensis; At: Arabidopsis thaliana; Al: Arctium lappa; Ha: Helianthus annuus; Nt: Nicotiana tabacum; Aa: Ageratina adenophora.

Phylogenetic analysis and multiple sequence alignment of C4Hs proteins (A) The phylogenetic tree containing C4H members from safflower and other plant species. The tree was constructed with MEGA 6.0 using the neighbor-joining (NJ) method and 1000 bootstrap replications. (B) Multiple sequence alignment of CtC4H1 and C4H from other plant species. The P450 conserved sequences are boxed in red, the underlined regions represent the six SRSs and Triangle represents E-R-R triad; Cb: Chrysanthemum boreale; Cs: Camellia sinensis; At: Arabidopsis thaliana; Al: Arctium lappa; Ha: Helianthus annuus; Nt: Nicotiana tabacum; Aa: Ageratina adenophora.