Plants ectopically expressing SPL13 and SPL9 are accelerated in vegetative phase change and mimic bop1 bop2 double mutant at seedling stage. (A, B) Ectopic expression of SPL13 in Col-0 and spl9/13 accelerated abaxial trichome production in both LDs (A) and SDs (B). (C) Sixteen-day-old Col-0, spl9 spl13, and rSPL13 plants in Col-0 or spl9 spl13 (spl9/13) plants growing in LDs. Scale bars: 3 mm. (D) RT-qPCR analysis of SPL13 transcripts in 12-day-old Col-0, spl9/13, rSPL13-GUS, rSPL13 Col-0, and rSPL13 spl9/13 seedlings. SPL13 is highly expressed in rSPL13-GUS, rSPL13 Col-0 and rSPL13 spl9/13 seedlings. Values are relative to Col-0 and represent the mean ±SEM from three biological replicates (red dots). Shared letters indicate not significantly different groups, different letters indicate significantly different groups; P<0.001, one-way ANOVA. (E) Twelve-day-old Col-0, rSPL13 plants, rSPL9 plants, and bop1 bop2 (bop1/2), 16-day-old rSPL13 plants, and 18-day-old rSPL9 plants growing in LDs. Note that the 16-day-old rSPL13 and 18-day-old rSPL9 plants mimic 12-day-old bop1/2. Scale bars: 3 mm.