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. 2023 Mar 28;6:334. doi: 10.1038/s42003-023-04708-2

Fig. 3. Harnessing CRISPR/RfxCas13d collateral activity for RNA-guided cell targeting.

Fig. 3

a–d HEK293T cells were transfected with RfxCas13d, DsRed, and either a DsRed-targeting gRNA or a non-targeting control gRNA. Shown are (a) cell proliferation (relative to control gRNA) as measured by WST-1 assay, (b) EdU incorporation assay, (c) nuclear morphology with DAPI staining, and (d) quantification of (c). e WST-1 assay for targeting endogenous mRNAs. (f), Selective depletion of DsRed-expressing U87GFP cells from a mixture of U87GFP and U87BFP cells by using RfxCas13d (stably expressed) and a control gRNA or DsRed gRNA (transfected on day 0). Two-way ANOVA was used for (a, e, f) and two-tailed Student’s t-test was used for (b, d). *p < 0.05; **p < 0.01. N = 3–4 for (a, b, e, f). 208 cells from the control group and 224 cells from DsRed-targeting gRNA group were measured for (d). Scale bar, 50 μm (b, c). Error bars represent standard deviation.