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. 2023 Mar 28;13:5072. doi: 10.1038/s41598-023-31781-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Photomicrograph of haematoxylin and eosin-stained sections from hippocampus of obese male rat (a–c) and obese female rat (d–f) treated with 30 mg of zinc showing dramatic improvement, where: (a) preservation of small pyramidal neurons with few apoptotic cells (↑); (b) some large pyramidal neurons with pyknotic nuclei (↑) and (c) preservation of granular cells with mild disorganization and vacuolation (V). Molecular layer (ML) mostly shows some enlarged neurons (↕) and many glial cells (wavy arrow); (d) preservation of small pyramidal cells (right pointing triangle) with few apoptotic cells (↑); (e) large pyramidal cells show few karyorrhectic nuclei (_*) and (f) preservation of granular cells (|) and the molecular layer (ML) mostly shows few enlarged neurons (↕) and many glial cells (wavy arrow) (H & E, ×400).